Trace Elements in Medicine (Moscow)
2019, 20(1): 13-20
ORIGINAL PAPERS

RESEARCH OF RICE MICROBIOCENOSIS OF RATS IN RESPONSE TO INTRODUCTION IN THEIR RATIONAL NANOPARTICLES OF COPPER AND ZINC

E.S. Aleshina¹, E.A. Drozdova¹, E.I. Tarasova¹, I.A. Gavrish^{1, 2}

¹ Orenburg State University, Victory ave. 13, Orenburg, 460018, Russia
² Federal Scientitfic Centre of Biological Systems and Agrotechnologies of Russian Academy of Science, 9 Janvarja str. 29, Orenburg, 460000, Russia

DOI: 10.19112/2413-6174-2019-20-1-13-20 

ABSTRACT. At present, to compensate for the imbalance of microelements in the body, a variant of using preparations based on nanopowders of metals is proposed. The purpose of this work was to study the effect of copper and zinc nanoparticles on the intestinal microflora of rats used as a source of a microelement. For the study, spherical nanoparticles were used. NPsCu was 0,51 mg / ml, NPsZn was 0,43 mg / ml. In the course of the study it was found that the introduction of metal nanoparticles into animal feed leads to the transformation of the intestinal microflora. So nanoparticles of metals have a harmful effect on the normoflora of the intestine. Throughout the experimental reduction, a sharp decrease in the representatives of the families Bifidobacteriaceae and Lactobacillaceae. At the same time, under the influence of nanoparticles, a conditionally pathogenic microflora is actively developing and against the background of a decrease in immune forces. However, in the process of research on day 14, the animal's organism gradually adapts to the elements introduced. An attempt was made to restore normal intestinal microflora, which is reflected in the prevention of excessive growth of pathogenic microflora. The observed inhibition of growth of bacteria of the family Staphylococcaceae, as well as conditionally pathogenic communities of the Enterobacteriacea family, such as E. coli and Salmonella. However, this is not enough to completely restore the normal intestinal microflora. The obtained results demonstrate that zinc and copper nanoparticles, even at low concentrations, lead to a change in the structure of the microbial community, stimulating the multiplication of individual groups of microorganisms and suppressing the normoflora of the intestine.

KEYWORDS: copper nanopowder, zinc nanopowder, essential trace elements, microbiota.