Trace Elements in Medicine (Moscow)
2017, 18(4): 30−35
ORIGINAL PAPER

EVALUATING THE EFFECTIVENESS OF CALCIUM USED IN MICELLAR FORM TO ACCELERATE BONE REPAIR AFTER FRACTURES

M.V. Stogov, E.N. Gorbach, M.A. Stepanov, N.V. Tushina

Russian Ilizarov Scientific Center «Restorative Traumatology and Orthopaedics», M. Ulyanova str. 6, Kurgan 640014, Russia

DOI: 10.19112/2413-6174-2017-18-4-30-35 

ABSTRACT. The study examined the effectiveness of micellar calcium compounds for accelerating bone fracture repair in the experimental model. The study was conducted on 20 adult mongrel dogs of both sexes. All animals modeled comminuted fractures of the tibia, followed by fixation of bone fragments by Ilizarov. All animals were randomly distributed into two groups. Animals of the first group (control, n = 10) in the posttraumatic period of additional actions aimed at enhancing the reparative osteogenesis did not receive. The animals of the second group (experiment, n = 10) in the post-traumatic period from the first day after the injury to the end fixation in drinking water were added 7 drops of the preparation "Miсellat-Active" diluted to 1 liter of drinking water. To evaluate the effectiveness of using clinical and radiographic, histological and biochemical methods. The animals were taken out of the experience at the time of the formation of bone fusion, and after 30 and 90 days after the termination of fixation. It was found that in the treated group a significant decrease in the level of calcium in the blood serum in the posttraumatic period revealed only on the 3rd day after the operation, in contrast to the control group, where hypocalcemia was maintained until the 14th day. Histological analysis revealed that the percentage of bone structures in a zone damaged site in the treated group at the end fixation was 31.4±1.4% (compact bone) and 49.5±2.1% (spongy bone) in control – 13.3±0.7 and 40.8±1.7%, respectively (differences were significant at p<0.05). According to X-ray electron microprobe microanalysis of the end locking dogs in the test group regenerate calcium zone averaged 11.70±0.31%, in the control – 1.07±0.03% (difference significant at p<0.05). The term fracture healing in the control group averaged 46.3±1.5 days in the experimental group – 40.1±3.1 days (differences significant at p<0.05). Thus, this study demonstrated the effectiveness of calcium used in micellar form in order to accelerate bone repair after fracture.

KEYWORDS: calcium, bone fracture