Trace Elements in Medicine (Moscow)
2017, 18(4): 13−17
SELENIUM REGULATION OXIDATION PROCESSES INDUCED BY SODIUM NITRITE IN THE BLOOD OF RATS
S.Y. Huseynova, T.M. Huseynov, R.T. Gulieva, F.R. Yahyaeva, M.Z. Dadashov, A.I. Jafarov
Institute of Biophysics of Azerbaijan NAS, Baku, Azerbaijan, Z. Khalilov str. 117, Baku, AZ1141, Azerbaijan
ABSTRACT. The role of selenium under the influence of moderate doses of sodium nitrite on rat erythrocytes in vivo was studied. Rats were exposed to separate and combined action of Na2SeO3 (0.5 mg/kg) and NaNO2 (30 mg/kg) by intraperitoneal injections and subsequent exposures with terms 1, 2, 3 and 12, 48 h. The introduction of sodium nitrite with exposures of 1 and 3 hours in the body of rats resulted in a noticeable accumulation of MetHb and already after one hour reached ≈ 30%, which monotonically decreased by 30% from the peak level within the next 2−3 hours. By 12 and 48 hours exposure, the MetHb level was not different from the control. Reduction (by 30% of benchmark) of products reacting with thiobarbituric acid (TBA) was revealed in the erythrocyte suspension under the influence of nitrite. The separated administration of sodium selenite did not change rate of MetHb and lipid peroxidation (LPO). In short exposure (1−3 hours), the combined administration of selenite and sodium nitrite decreased the nitrite-induced accumulation of MetHb by ≈ 35% and increased the accumulation of LPO products in comparison with version of influence only nitrite. In this case, the sequencing of introduction did not affect the final result. During a long exposure, the previously introduced selenite (exposure of 48 h), with subsequent introduction of nitrite (1 h incubation), decreased the nitriteinduced accumulation of MetHb by ≈16% and LPO values by 41%, but selenite (48 h exposure ) introduced in 1 h after nitrite, did not affect the accumulation of MetHb and slightly (10%) reduced LPO values. The change in the activity of antioxidant (AO) enzymes glutathione peroxidase (GPx) and catalase was also considered. The catalase activity decreased under all exposure options of sodium nitrite. Selenite during short exposure did not lead to a marked increase of the activity of GPx, and nitrite led to its inhibition. Combined with nitrite effect of selenite had little impact on the NaNO2-induced reduction of GP activity. The decrease of the nitrite-induced accumulation of MetHb, during the administration of sodium selenite in the first 1−3 hours, probably more associated with the fact of the inclusion of selenium in the Hb molecule, than additional contribution of GP, which activity is not significantly increased in the indicated period. Based on the position of the spectral maxima for HbO2 and doxHb, it can be concluded that NaNO2 increases MetHb through a decrease in HbO2, and selenite inhibits this effect to a certain extent.
KEYWORDS: sodium nitrite, sodium selenite, erythrocytes, methemoglobin, glutathione peroxidase, catalase, lipid peroxidationCorresponding author: Huseynov T.M.; E-mail: firstname.lastname@example.org
© Trace Elements in Medicine (Moscow), 2017