Trace Elements in Medicine (Moscow) 2016, 17(3): 41-47

DEVELOPMENT OF MEDICAL PRODUCTS ON THE BASIS OF OLIGOGALACTURONIC ACID, AS CARRIERS OF BIOGENIC METALS (II) AND HEAVY METAL (II) ANTIDOTES

N.Sh. Kajsheva, A.Sh. Kajshev

Pyatigorsk Medico-Pharmaceutical Institute, Kalinin ave. 11, Pyatigorsk, 357532, Russia

DOI: 10.19112/2413-6174-2016-17-3-41-47 

ABSTRACT. Medicinal preparations on the basis of oligogalacturone acid (OGA) were proposed as carriers of biogenic metals (BMe) (II) (offered for Cu, Co, Fe, Zn, Mn, Mg) and heavy metal (HMe) (II) antidotes (offered for Pb, Cd). Being obtained from industrial beet pectin, suitable for medical use, the OGA is an acid with molar weight 3200 g/mol and the polymerization degree of 18. Its рН (3.5) was lead up to 8.0 by means of 2.0 mol/l solution of ammonia hydrate. Further, 5.0 mmol/l solution of the acid was mixed with about 45.0 mmol/l solution of acetate of one of divalent metals: Cu, Co, Fe, Zn, Mn, Mg in an identical volumetric parity. The mix were subsequently processed by 20.9 mol/l ethanol (1:2), kept at 20°С within 2 hours, neutralized (acetic acid is a product of reaction) by 2.0 mol/l solution of ammonia hydrate, separated and dried at 60°С. As the criteria for experimental selection of optimum conditions for producing oligogalacturonates of biogenic metals (OGBMe) (II) there were used: completeness of the complexes formation (constants of balance 106), absence of precipitated surplus of the metal ions, stability of products at рН 8 (constant of stability 109), technological output. The choice of beet pectin as the source of OGA was reasoned by its high ability to form complexes: 23.55 mole of Pb2+ ions on 1 mole of the pectin. The choice of OGA as the carrier of metals (II) was reasoned by its high biological availability and ability to accumulation in biological liquids and fabrics, especially bone fabric, being a place of heavy metals deposition. The degrees of BMe (II) desorption from corresponding OGMe in Pb2+ and Cd2+ solutions (80,0%) and absorption of Pb2+ and Cd2+ from them (78,0%) testify a possibility to use OGA for delivery of BMe (II) and removal of HMe (II). Experiments on a model of anemia in animals intoxicated by lead (II) acetate were proved the ability of OGFe2+ to increase the quantity of erythrocytes 1.6 times, the content of hemoglobin in blood 1,8 times and to reduce the content of Pb2+ ions in blood 2 times.

KEYWORDS: biogenic metals, heavy metals, antidotes, carriers of metals.