TRACE ELEMENTS IN MEDICINE (MOSCOW)
2015. 16(4): 28-35
ORIGINAL PAPERS

THE ROLE OF SELENIUM IN THE MULTIVARIATE ETIOPATHOGENESIS OF ISCHEMIC STROKE

L.L. Klimenko1, A.V. Skalny2, A.A. Turna3, A.V. Kuznetsova6, O.V. Senko4, I.S. Baskakov1, M.N. Budanova1, M.S. Savostina5, A.N. Mazilinа5

1 N.N. Semenov Institute of Chemical Physics, Russian Academy of Sciences, Kosygin str. 4, Moscow, 119991, Russia; e-mail: klimenkoll@mail.ru
2 Center for Biotic Medicine, Zemlyanoi val 46, Moscow, 105064, Russia
3 Institute of Professional Development of FMBA of Russia, Volokolamskoe shosse 30, Moscow, 123182, Russia
4 Computing Centre of the Russian Academy of Sciences, Vavilov str. 40, Moscow, 119333, Russia
5 Hospital № 123 at FMBA of Russia, Krasnogorskoe shosse 15, Odintsovo, Moscow region, 143000, Russia
6 N.M. Emanuel Institute of Biochemical Physics, Russian Academy of Sciences, Kosygin str. 4, Moscow, 119334, Russia

DOI: 10.19112/2413-6174-2015-16-4-28-35 

ABSTRACT. The role of selenium in the multivariate etiopathogenesis of ischemic stroke was analyzed. In clinical settings, biomarkers of dyscirculatory pathology was assessed in patients diagnosed with «ischemic stroke» (n = 96, mean age 65 ± 0.3 years). Selenium concentration in the blood serum (µg/ml) was determined by atomic emission spectrometry with inductively coupled argon plasma. Selenium is a cofactor of glutathione antioxidant enzyme, and its concentration is an indicator of the antioxidant protection activity. Concentration of neurospecific proteins, antibodies to the NR2 peptide (NR2-AB, ng/ml), was determined in blood serum by ELISA. Their level is strictly a predictor of ischemic stroke that characterizes the extent of damage of ishemic brain tissue. Brain DC-potentials were recorded by a non-invasive neurophysiological method in different age groups. Mann-Whitney method showed significantly lower concentrations of selenium in the oldest age group (over 60 years) that indicates depletion of glutathione antioxidant enzymes in the later age. A contingency between concentrations of selenium and NR2-AB was found: the higher the selenium concentration, the lower the concentration of NR2-AB, and vice versa. This fact confirms the interdependence of the antioxidant protection, reflected by selenium, and the neurospecific proteins NR2-AB which are the indicator of ischemic brain tissue damage. Correlation analysis revealed a significant association between the concentration of selenium and the DC-potential in different areas of the brain as well as between the concentrations of selenium and NR2-AB, thus suggesting the possibility of using these indicators as biomarkers of cerebral ischemia in order to ensure maximum safety of brain tissue atdyscirculatory disorders.

KEYWORDS: ischemic stroke, selenium, NR2-antibodies, energy metabolism, DC-potential, trace elements.