ABSTRACT.
Objective – investigation of selenium (Se) metabolism characteristics in patients with knee osteoar-thritis (OA) depending on the presence of comorbid hip osteoarthritis, with assessment of Se levels in biosamples, as well as selenoprotein P (SELENOP) level and glutathione peroxidase (GPX) аactivity in serum.
Materials and methods. During the study 27 patients with knee OA, 45 patients with comorbid knee and hip OA, as well as 37 healthy subjects were examined. Assessment of serum Se was performed using inductively-coupled plasma mass-spectrometry. Evaluation of serum SELENOP, GPX, and cartilage oligomeric matrix protein (COMP) was per-formed using enzyme-linked immunosorbent assay. Assessment of C-reactive protein (CRP) levels was performed us-ing immunoturbidimetry.
Results. Serum COMP and CRP levels in patients with knee OA and comorbid knee and hip OA exceeded the control values by 123% and 99%, as well as 69% and 127%, respectively. Similarly, hair Se levels and serum GPX concentration in these groups of patients was higher compared to the respective values in healthy controls by 73% and 41%, as well as 31% and 38%. Only an insignificant trend to a decrease in serum Se levels was observed. At the same time, serum concentration of SELENOP, being the major Setransporting protein in blood, in patients with knee OA and comorbid knee and hip OA was lower than that in healthy examinees by 18% and 45%, respectively.
Conclusions. It is assumed that an increase in serum GPX levels may be a compensatory response to oxidative stress in cartilage damage, whereas a reduction in serum SELENOP in parallel with a trend to lower circulating Se concentration, may be indicative of the formation of Se deficiency in OA due to higher demands of the organism in Se for biosynthesis of antioxidant selenoproteins like GPX.
KEYWORDS: selenium, selenoproteins, osteoarthritis, knee, hip.
For citation: Skalny A.V., Korobeinikova T.V., Aliyev A.F., Remizova N.I., Morozova G.D., Ternovoy K.S., Kovalenko A.A., Tinkov A.A. Serum selenoprotein P level, glutathione peroxidase activity, and other markers of selenium metabolism in patients with osteoarthritis. Trace elemets in medicine. 2025;26(4):15-24. DOI: 10.19112/2413-6174-2025-26-4-15-24
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Information about the authors:
Anatoly V. Skalny – Dr.Sc. (Med.), Professor, Director of the Center for Bioelementology and Human Ecology1;
Head of the Department of Medical Elementology2
E-mail: avskalny@gmail.com; ORCID: 0000-0001-7838-1366
Tatiana V. Korobeinikova – Ph.D. (Tech.), Head of the Laboratory of Molecular Dietetics,
Center for Bioelementology and Human Ecology1; Associate Professor of the Department of Medical Elementology2
E-mail: tatcvetk@yandex.ru; ORCID: 0000-0002-1373-6354
Ali F. Aliyev – Traumatologist Orthopedist, Department of Medical Rehabilitation, University Clinical Hospital № 2
E-mail: dr.aliev90@gmail.com
Nelli I. Remizova – Physiatrist, Department of Medical Rehabilitation, University Clinical Hospital № 2
E-mail: nelly13m@mail.ru
Galina D. Morozova – Laboratory Assistant, Laboratory of Molecular Dietetics,
Center for Bioelementology and Human Ecology
E-mail: morozova0826@gmail.com; ORCID: 0000-0001-8600-902X
Konstantin S. Ternovoy – Ph.D. (Med.), Head of Department of Medical Rehabilitation,
University Clinical Hospital № 2
E-mail: Ternovoy_k_s@staff.sechenov.ru
Alexey A. Kovalenko – Ph.D. (Med.), Director of University Clinical Hospital № 2,
Associate Professor of the Department of Sports Medicine and Medical Rehabilitation
E-mail: alexey-kovalenko@yandex.ru
Alexey A. Tinkov – Dr.Sc. (Med.), Principal Investigator of the Center for Bioelementology and Human Ecology1;
Professor of the Department of Medical Elementology2
E-mail: tinkov.a.a@gmail.com; ORCID: 0000-0003-0348-6192
Conflict of interest
The authors declare no obvious and potential conflicts of interest related to the publication of this article.
Funding
The study was performed with the financial support of the Russian Science Foundation (project No. 24-45-00073).
Поступила 10 ноября 2025 года
Принята к публикации 1 декабря 2025 года