ABSTRACT.
The objective of the present study was to identify the features of the structure of the parotid salivary
gland and mineral metabolism of male adolescent rats during lead intoxication.
Materials and methods. The object of the study is the parotid salivary glands (PSG) of 4-week-old males. 2
groups of 10 animals were formed: control males and rats with administration of Pb acetate solution 1 time per day for 3
weeks. Pb acetate was previously dissolved in water so that 1.0 mg of lead per 0.2 ml of solution. To ensure a dosage of
10 mg / kg of weight, the animals were weighed before feeding and, in accordance with the weight, the necessary
amount of toxicant solution was injected into the cracker with a syringe. Clean water was introduced into the control.
PSG was performed according to the generally accepted method for filling into histomix. Morphometry was performed
under a light microscope at 400 magnification using an 88-point grid. The areas of cytoplasm and epithelial cell nuclei,
duct lumen, connective tissue around the duct and hemocapillaries in it were determined in the excretory ducts. To
measure the diameter and lumen of the end sections of the OSJ, the ImageJ 1.54d program was used. In skeletal muscle,
the determination of Ca, Cd, Mg, Mn, P, Pb, Se, Zn, K was performed by ICP-MS.
Results. With the accumulation of Pb, the diameter of the OSJ acinuses grows by 12.39%, and the diameter of their lumen by 38.1%. The area of the epithelium of the ducts is reduced by 23.19% due to the cytoplasm. The lumen of the duct grows by 35.98%. Interstitial spaces around the duct expand by 16.4%, due to connective tissue by 37.7%, the proportion of hemocapillaries decreases by 41.11%. The amount of bioelements in the muscle drops by 2 or more times.
Conclusions. Changes in the structure of the OSH and the amount of bioelements during the accumulation of Pb reflect impaired secretion and passage of saliva, which leads to a change in the chemical composition of oral fluid and homeostasis in the oral cavity.
KEY WORDS: lead acetate, adolescent males, parotid salivary glands, mineral metabolism.
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