Supplement to the journal
"Trace Elements in Medicine" (Moscow)
2021: 54-55

VARIATION IN THE CONTENT OF CHLOROPHYLL IN WHEAT UNDER THE INFLUENCE OF VARIOUS FORMS OF IRON

E.A. Osipova¹*, S.V. Lebedev^1,2

¹Orenburg State University
² FSSI "Federal Research Center of Biological Systems and Agro-technologies of the Russian Academy of Sciences"

DOI: 10.19112/2413-6174-2021-S1-27 

ABSTRACT. The purpose of this study was to determine the content of chlorophyll in wheat scions growing under the influence of various forms of iron (Fe⁰ (80 ± 5 nm), Fe₃O₄ (width 50–80 nm, height 4–10 nm), FeSO₄•7Н₂О, Fe₂ (SO₄)₃•9Н₂О). The pigments were extracted using 96% ethyl alcohol on day 7; the chlorophyll concentration was calculated using the Vernon equations.The maximum synthesis of chlorophyll a and b was typical for iron ionic forms at a concentration by 1∙10^-3 g/l and iron nanoparticles at a concentration by 1∙10^-4 g/l, and Fe₃O₄ magnetite at a concentration of 1∙10^-6 g/l. The ratio of chlorophyll a to chlorophyll b in the terrestrial part of wheat on day 7 corresponds to the norm using iron nanoparticles Fe⁰ and ferrous iron ions in the form of FeSO₄ . As the concentration of magnetite nanoparticles increases, the concentration of chlorophyll a increases, and when the maximum concentration amount∙10^-3 g/l, the amount of chlorophyll b rise, while the a/b ratio decreases.In such a way, iron Fe⁰ nanoparticles and an aqueous solution of iron (II) sulfate contribute to the formation of a photosynthetic apparatus with intensive photosynthesis.

KEYWORDS: wheat, nanoparticles, iron, magnetite, iron sulfate, chlorophyll.