Trace Elements in Medicine (Moscow)
2018, 19(2): 41-48
ORIGINAL PAPER

DIAGNOSTIC ASSESSMENT OF SENSITIZATION TO MICROELEMENTS IN CHILDREN WITH BRONCHOPULMONARY PATHOLOGY

G.P. Evseeva, S.V. Suprun , T.V. Pivkina, E.D. Tselykh , O.I. Galiant, E.V. Knizhnikova, N.M. Ershova, L. M. Gretchenko

1Khabarovsk Branch of Far Eastern Scientific Center of Physiology and Pathology, of Respiration Research Institute of Maternity and Childhood Protection, 49 Voronezhskaya Str., Khabarovsk, 680022, Russia 2 Federal State Budget Educational Institution of Higher Education «Far Eastern State Transport University» Institute of Natural Sciences, 47 Serysheva Str., Khabarovsk, 680021, Russia 3 Territorial State Budgetary Health Institution «Children municipal polyclinic №17», 177Б Krasnorechenskaya Str., Khabarovsk, 680023, Russia

DOI: 10.19112/2413-6174-2018-19-2-41-48 

Pb, Mn, Ni in children with bronchopulmonary pathology is proposed in the article. 30 healthy children (control group), 25 children with acute respiratory problem, 122 children with chronic nonspecific bronchopulmonary pathology (66 children with asthma and 56 children with congenital abnormalities of the lungs) were examined. The average age of the children was 8.12±0.45 years. The content of trace elements in children's hair was determined by the method of mass spectrometry with inductively coupled plasma. For determination of hypersensitivity to salts of metals the neutrophils damage index (NDI test) in vitro was used (Hotchkiss with simplification of R.P. Nartsissov (1985). Solutions of chemical compounds were used (Zinc Sulphate – ZnSO4•7H2O, Nickel Sulphate – NiSO4, Manganese Sulfate – MnSO4•5H2O, Lead Nitrate – Pb(NO3)2). The selection of metal salts concentrations was carried out empirically. Salts concentrations not causing hemolysis and degenerative changes of red blood cells, as well as toxic changes in white blood cells during a two-hour incubation of blood of healthy individuals were considered desirable. The result is given in the form of an index “test indicator to control indicator”. In the control group sensitization to Nickel was detected in 10% of cases, to Lead and Manganese – in 6,7% of cases. No higher than normal index of neutrophils damage to Zinc in healthy children was detected, in contrast to children with acute and chronic bronchopulmonary pathology. Thus, the frequency of high neutrophils damage index to Nickel was detected in 32.4–31.1% of cases, to Lead – in 16.3–28.7% of cases, to Manganese – in 7.1–16.1% of cases, to Zinc – in 8.1–17.2% of patients with bronchopulmonary pathology. NDI test conduction can be a useful additional tool for the detection of allergy to metals in vitro, eliminating the contact of the allergen with the body, side effects and health complications after its injection

KEYWORDS: children, bronchopulmonary pathology, microelements, sensitization, neutrophils damage index.