Trace Elements in Medicine (Moscow)
2017, 18(3): 30–35
ORIGINAL PAPER

EPR QUANTIFICATION OF NITROXIDE IN EPIDERMIS ON THE BACKGROUND OF MILDRONATE TAKING: CORRELATION WITH SERUM FERRITIN LEVEL

V.I. Petukhov1, L. Baumane2, I. Kalvinsh2, M.A. Romanova1, E. Bykova3, L. Hemij3

1 Vladimir State University, Gorky str., 87, Vladimir, 600000, Russia
2 Latvian Institute of Organic Synthesis, Aizkuraukles str., 21, Riga, LV1006, Latvia
3 Riga Reproduction Centre, Antonijas str. 8, 1. st., Riga, LV1010, Latvia

DOI: 10.19112/2413-6174-2017-18-3-30-35 

ABSTRACT. The article reports on the results of a quantitative estimation of nitric oxide (NO) level in the deriva-tives of epidermis (hair) in 34 volunteers (4 men and 30 women aged 19 to 78 years) while taking Mildronate. Meas-urements were made by the Electron Paramagnetic Resonance (EPR) method using diethylthiocarbamate (DETC) in vitro (as a «trap»). For 116 subjects (32 males and 84 females aged 17 to78), we analysed the correlation (Pearson) be-tween the NO-radical signal intensity on the spectrogram and the serum ferritin level (as a body iron level criterion). It was found that Mildronate administration resulted in a significant increase of NO-peak height on the EPR spec-trogram in apparently healthy subjects. The NO-peak height showed positive correlation with the serum ferritin level.

KEYWORDS: nitric oxide (NO), EPR spectroscopy, Mildronate, Fe deficiency.