TRACE ELEMENTS IN MEDICINE (MOSCOW)
2016, 17(2): 45-50
ORIGINAL PAPERS

INFLUENCE OF SYNTHETIC SELENIUM COMPOUNDS ON ERYTHROPOIESIS AT EXPERIMENTAL LEAD INTOXICATION

К.О. Sharipov1*, К.К. Оmirzakova1, К.А. Bulygin1, R.F. Yakhin1, A.A. Batyrbayeva2, A.Zh. Kerimkulova2, G.M. Tleshova2

1 Asfendiyarov Kazakh National Medical University, Tole Bi str. 94, Almaty 050000, Kazakhstan
2 Satpayev Kazakh National Research Technical University, Satpayev str. 22, Almaty 050013, Kazakhstan

DOI: 10.19112/2413-6174-2016-17-2-45-50 

ABSTRACT. Influence of new synthetic selenium derivatives of different structure on the basic parameters of the peripheral red blood while acute lead intoxication was studied in rabbits. Sodium selenite was taken as the control substance. The study was conducted on outbred rabbits of both sexes. The acute lead intoxication was modeled by intragastric injection of lead acetate water solution in a dose of 120 mg/kg. The animals were divided in the following groups: intact, control and experimental. The experimental group simultaneously with lead intoxication was administered by injections of selenium preparations in an experimentally established active therapeutic dose for 14 days. As a comparison preparation the sodium selenite was taken. The selenium compounds had the following structure: 1,2,5-dimethyl-4,4-di(butylseleno)piperidine (BSP); (2,3-diazo-1-seleno)-1,2,3-trimethyl piperidine-3en (DSP-2); piperidine-(dibutilseleno)-selenosulfat (PSF). Their structure was proved by IR, PMR-spectroscopy and the composition was proved by elemental microanalysis. We have noted that during lead intoxication significant violations occurred in the red blood cells; they were developed in a certain sequence: at the beginning reticulocytosis and basophilic stippling of red blood cells appeared, later – anemia with a maximum reduction of hemoglobin.
During the work it was found that the studied selenium compounds significantly normalize erythropoiesis, restore hemoglobin levels, reduce reticulocytosis and basophilic granularity of red blood cells. It was shown that the tested organic preparations are not less effective than sodium selenite and in some cases it surpasses the sodium selenite. An antianemic effect of the BSP comparable with sodium selenite is supposed to be due to the presence of two atoms of selenium in its composition, high toxicity and absent influence of organic part of the molecule on hemoglobin metabolism and erythropoiesis. A positive effect of the DSP -2 and PSP on red blood parameters superior to that of sodium selenite in this model of intoxication is probably explained by influence of organic parts of the molecules after their destruction on conjugated processes in the blood regeneration system by several mechanisms.

KEYWORDS: Organoselenium compounds, lead intoxication, antianemic effect, erythrocytes, hemoglobin.