TRACE ELEMENTS IN MEDICINE (MOSCOW)
2015. 16(1): 41-47
ORIGINAL PAPERS

THE DYNAMICS OF ELEMENT REDISTRIBUTION IN ORGANS AND TISSUES OF WISTAR RATS IN THE EXPERIMENT WITH A LOAD OF MANGANESE CHLORIDE

М.S. Goncharenko, A.V. Goncharenko, H.P. Andreyko, T.M. Chykalo

V.N. Karazin Kharkiv National University, Maidan Svobody 4, Kharkiv, 61022, Ukraine; e-mail: valeolog@univer.kharkov.ua

DOI: 10.19112/2413-6174-2015-16-1-41-47 

ABSTRACT. According to the literature data it was established that in a number of ecological residential areas with increased content of manganese, there is an increased amount of this microelement in human organisms, which is accompanied by a number of diseases. Due to insufficient knowledge on the biological mechanisms of action of manganese hyperelementosis, a modeling was conducted to study the influence of toxic load of manganese chloride at doses of 25, 30 and 50 mg/kg wet weight in seven days on the redistribution of Ca, Mg, Zn, Cu and Mn in organs and tissues of albino rats. The mineral composition of the liver, kidneys, muscles, bones, heart and spleen of white rats examined after sample preparation conducted by atomic absorption spectroscopy with a spectrophotometer C–115 M1. Comparison of the control and experimental groups revealed that manganese hyperelementosis causes a significant redistribution of macro- and trace elements in organs and tissues of the animals and change their total elemental content. The dose 25 mg/kg causes an increase in the total content of the elements and their redistribution that can be interpreted as a protective adaptive response to maintain ion homeostasis. At the dosage 50 mg/kg we observed a significant reduction in the total content of elements at a considerable decrease in the amount of magnesium, zinc, copper, manganese and a significant increase in all studied organs and tissues. Perhaps, such element redistribution could be explained by the results previously established in biochemical mechanisms of the effect of high manganese content on the functional properties of the tissue, which are caused by antagonistic behavior of this trace element to Mg, Zn and Cu and are expressed in violation of the barrier and transport properties of cell membranes and reducing the energy efficiency of the mitochondrial respiratory chain. Direction of dynamics of the detected changes is determined by the administered dose of manganese.

KEYWORDS: hyperelementosis, manganese, macroelements, trace elements, redistribution, total content.