ABSTRACT.
Until recently, elemental homeostasis was assessed by the total content of chemical elements in various biosubstrates, however, recent studies have shown that changes in the level of trace elements, even in the range of normal values, are accompanied by their redistribution to various protein fractions. In this regard, the purpose of this study was to study the effect of a high-calorie diet on the metabolism of trace elements, taking into account the study of chemical forms of copper
in blood serum. The animals were divided into two groups: group I (control) received a general diet, group II (experimental)
received a high-calorie diet. The total diet had a calorie content of 270 kcal/ 100 g. A high-calorie diet is based on the addition
of lard, coconut and sunflower oils to the general diet, fructose was added to the water. Over the course of 12 weeks, the caloric content increased stepwise by 30%, 60% and 90% of the caloric content of the total diet. The analysis of copper forms was
performed by dividing blood serum into fractions, followed by determination of the metal content in each fraction by inductively coupled plasma mass spectrometry. The separation was carried out using a system for high-performance liquid chromatography. In the experimental group, an increase in the level of the transcuprein fraction of copper from 8% to 24%, low molecular forms of copper from 5% to 15% was revealed against the background of a decrease in the ceruloplasmin fraction of
copper from 65% to 43% and the albumin fraction of copper from 22% to 18%. Evaluation of copper fractions in blood serum
revealed the presence of metabolic disorders of this metal with a high-calorie diet at the stage preceding significant changes in
the total concentration in blood serum.
KEYWORDS: trace elements, high-calorie diet, rats, copper.