Trace Elements in Medicine (Moscow)
2021, 22(3): 47-57
ORIGINAL PAPERS

INFLUENCE OF ENRICHMENT OF THE RAT DIET WITH INULIN ON THE ASSIMILATION OF CERTAIN VITAMINS AND MINERALS

N.A. Beketova, V.M. Kodentsova, S.N. Leonenko, O.V. Kosheleva, O.A. Vrzhesinskaya, S.Kh. Soto, A.A. Sokolnikov, L.V. Shevyakova, N.V. Zhilinskaya

Federal Research Centre of Nutrition, Biotechnology and Food Safety, 2/14, Ustyinskiy proezd, Moscow, 109240, Russian Federation

DOI: 10.19112/2413-6174-2021-22-3-47-57 

ABSTRACT. Inulin is widely used in the food industry as a source of food soluble dietary fiber and a prebiotic. However, its effect on the absorption of vitamins has not been studied enough. The effect of introducing soluble dietary fiber (5% inulin) into the diet on the correction of vitamin D and group B deficiency and its consequences in growing male Wistar rats (an initial body weight of 51.4 ± 0.5 g) after a deficiency due to a decrease in the content of vitamins D and group B in the vitamin mixture during 23 days. The concentration of vitamins A in blood plasma and lyophilized liver and the whole brain of rats was determined by HPLC, vitamins B1 and B2 in the liver, brain, urine and 4-pyridoxic acid in urine - by fluorimetric method. Biochemical parameters of blood and urine were determined using a biochemical analyzer or KoneLab 200i (Ther-moScientific, Finland). The enrichment of the diet of rats with inulin did not affect the assimilation of vitamins A and D by deficient animals during the 7-day correction of the vitamin status, somewhat slowed down the restoration of the normal supply of vitamins B1, and B6 (by urinary excretion), B2 (by the content in the brain). At the same time, the content of vitamin E in the liver was reduced by 1.48 times compared with the control group, the concentration of iron in the blood plasma was increased by 32.7%, in the liver  by 42.6%, the content of manganese in the brain was increased by 1.5-fold, without going beyond the physiological norm. This indicates the advisability of enriching the diet of persons suffering from iron deficiency with inulin while enriching the diet with vitamins E and group B.

KEYWORDS: multiple deficiency of vitamins D and B group; inulin, correction, α-tocopherol, iron, manganese, rats.