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Trace Elements in Medicine
International scientific and practical peer-reviewed journal
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VARIATION IN THE CONTENT OF CHLOROPHYLL IN WHEAT UNDER THE INFLUENCE OF VARIOUS FORMS OF IRON

Supplement to the journal
"Trace Elements in Medicine" (Moscow)
2021: 54-55

VARIATION IN THE CONTENT OF CHLOROPHYLL IN WHEAT UNDER THE INFLUENCE OF VARIOUS FORMS OF IRON

E.A. Osipova1*, S.V. Lebedev1,2

1Orenburg State University
2 FSSI "Federal Research Center of Biological Systems and Agro-technologies of the Russian Academy of Sciences"

DOI: 10.19112/2413-6174-2021-S1-27 

ABSTRACT. The purpose of this study was to determine the content of chlorophyll in wheat scions growing under the influence of various forms of iron (Fe0 (80 ± 5 nm), Fe3O4 (width 50–80 nm, height 4–10 nm), FeSO4•7Н2О, Fe2 (SO4)3•9Н2О). The pigments were extracted using 96% ethyl alcohol on day 7; the chlorophyll concentration was calculated using the Vernon equations.The maximum synthesis of chlorophyll a and b was typical for iron ionic forms at a concentration by 1∙10-3 g/l and iron nanoparticles at a concentration by 1∙10-4 g/l, and Fe3O4 magnetite at a concentration of 1∙10-6 g/l. The ratio of chlorophyll a to chlorophyll b in the terrestrial part of wheat on day 7 corresponds to the norm using iron nanoparticles Fe0 and ferrous iron ions in the form of FeSO4 . As the concentration of magnetite nanoparticles increases, the concentration of chlorophyll a increases, and when the maximum concentration amount∙10-3 g/l, the amount of chlorophyll b rise, while the a/b ratio decreases.In such a way, iron Fe0 nanoparticles and an aqueous solution of iron (II) sulfate contribute to the formation of a photosynthetic apparatus with intensive photosynthesis.

KEYWORDS: wheat, nanoparticles, iron, magnetite, iron sulfate, chlorophyll.

Corresponding author: E.A. Osipova е-mail: kudryavceva.elen@mail.ru