Supplement to the journal
"Trace Elements in Medicine" (Moscow)
VARIATION IN THE CONTENT OF CHLOROPHYLL IN WHEAT UNDER THE INFLUENCE OF VARIOUS FORMS OF IRON
E.A. Osipova1*, S.V. Lebedev1,2
1Orenburg State University
2 FSSI "Federal Research Center of Biological Systems and Agro-technologies of the Russian Academy of Sciences"
ABSTRACT. The purpose of this study was to determine the content of chlorophyll in wheat scions growing under the influence of various forms of iron (Fe0 (80 ± 5 nm), Fe3O4 (width 50–80 nm, height 4–10 nm), FeSO4•7Н2О, Fe2 (SO4)3•9Н2О). The pigments were extracted using 96% ethyl alcohol on day 7; the chlorophyll concentration was calculated using the Vernon equations.The maximum synthesis of chlorophyll a and b was typical for iron ionic forms at a concentration by 1∙10-3 g/l and iron nanoparticles at a concentration by 1∙10-4 g/l, and Fe3O4 magnetite at a concentration of 1∙10-6 g/l. The ratio of chlorophyll a to chlorophyll b in the terrestrial part of wheat on day 7 corresponds to the norm using iron nanoparticles Fe0 and ferrous iron ions in the form of FeSO4 . As the concentration of magnetite nanoparticles increases, the concentration of chlorophyll a increases, and when the maximum concentration amount∙10-3 g/l, the amount of chlorophyll b rise, while the a/b ratio decreases.In such a way, iron Fe0 nanoparticles and an aqueous solution of iron (II) sulfate contribute to the formation of a photosynthetic apparatus with intensive photosynthesis.
KEYWORDS: wheat, nanoparticles, iron, magnetite, iron sulfate, chlorophyll.Corresponding author: E.A. Osipova е-mail: email@example.com