Trace Elements in Medicine (Moscow)
2017, 18(4): 18−21
ORIGINAL PAPER

STUDY OF THE INFLUENCE OF DINITROSYL IRON COMPLEXES ON SOME PHYSICAL AND CHEMICAL PROPERTIES OF RATS’ BLOOD SERUM

A.K. Martusevich 1,2, L.K. Kovaleva 2, A.A. Martusevich 3

1 Privolzhsky Federal Medical research Centre, Verhnevolzhskaya emb., 18/1, Nizhny Novgorod 603159, Russia
2 Kirov State Medical University, Karl Marx st., 112, Kirov 610027, Russia
3 National Research Nizhny Novgorod State University named after N.I. Lobachevsky, Gagarin av., 23, Nizhny Novgorod 603950, Russia

DOI: 10.19112/2413-6174-2017-18-4-18-21 

ABSTRACT. The aim of this work was estimation of dinitrosyl iron complexes (DNIC) influence on own crystallization of blood serum of the rats. Our experiment was carried out on 60 male Wistar rats, divided into 6 equal groups. First group was intact (without any manipulations). Rats of other groups got a course of intraperitoneal administration of 1 ml. of saline during 10 days. For rats of third to sixth groups saline additionally contained the dinitrosyl iron complexes with glutathione ligands (concentration – 0.15; 0.30; 0.45 and 0.60 мМ, respectively). Crystallogenic properties of rats’ blood serum were tested by classic crystalloscopy method with calculation of special semiqualitative criteria. They included crystallizability, structure index, facia destruction degree and clearity of marginal zone and were estimated in four-point scale. It was stated that glutathione-containing DNIC has a proactivating effect on crystallogenic potential of blood serum of healthy rats. This tendency realized in elevation of crystals density to control and intact groups. We also observed the appearance of numerous dendrite elements in facias of blood serum in rats injected with DNIC solution. Facia destruction degree in biological fluid specimens was elevated moderately in rats of main groups. Clearity of marginal zone is indicating the state of blood serum proteome was registered in constant level. Maximal changes of all parameters was fixed at the use of 0.3 and 0.45 mM of DNIC.

KEYWORDS: nitric oxide, dinitrosyl iron complexes, crystallization, blood serum