Trace Elements in Medicine (Moscow)
2017, 18(1): 17–21
ORIGINAL PAPER

ESTIMATION OF HAIR TRACE ELEMENT CONTENT IN RATS WITH NON-ALCOHOLIC FATTY LIVER DISEASE

E.R. Gatiatulina1,2, E.V. Popova1, V.S. Polykova1, А.А. Skalnaya3, E.F. Agletdinov4, A.A. Nikonorov1,5, A.V. Skalny 5,6,7,8, A.A. Tinkov1,5,6,7

1 Orenburg State Medical University, Sovetskaya St., 6, Orenburg, 460000, Russia
2 South Ural State Medical University, Vorovskogo St., 64, Chelyabinsk, 454048, Russia
3 Lomonosov Moscow State University, Lomonosovsky Prospekt, 31-5, Moscow, 117192, Russia
4 Bashkir State Medical University, 3, Lenin Street, Ufa, 450008, Russia
5 Orenburg State University, Pobedy avenue, 13, 460018, Orenburg, Russia
6 Yaroslavl State University, Sovetskaya St., 14, Yaroslavl, 150000, Russia
7 RUDN University, Miklukho-Maklay St., 10/2, Moscow, 117198, Russia
8 All-Russian Research Institute of Medicinal and Aromatic Plants (VILAR), Grina St., 7, Moscow, 117216, Russia

DOI: 10.19112/2413-6174-2017-18-1-17-21 

ABSTRACT. Non-alcoholic fatty liver disease (NAFLD) is the commonest liver disease in the world associated with insulin resistance, obesity, type 2 diabetes mellitus and metabolic syndrome. The prevalence has been estimated to be approximately 25-30% in the Western countries and USA and about 27% in Russian Federation. It is supposed that essential and toxic trace elements may play a significant role in NAFLD development. Consequently, the aim of the study was to assess the influence of diet-induced NAFLD on hair trace elements content of rats. Totally, 56 female 3-month Wistar rats were used in the present study. Rats were divided into two groups (n=28) and fed either standard (10% fat, SD = control) or high-calorie diet (60% of fat, HCD = NAFLD) during 6 weeks, respectively. Hair trace element content was assessed by means of inductively coupled plasma mass spectrometry (ICP-DRC-MS). It was revealed that high calorie feeding resulted in a significant increase in hair V concentration in animals. Oppositely, Cu, I, Li, and Zn levels in NAFLD rats were decreased in comparison to the control values. There was no significant difference in hair Co, Cr, Fe, Mn, and Se content between the studied groups. Toxic trace element (Al, As, Cd, Ni, and Sr) levels also did not affected by the NAFLD. In conclusion, the obtained data demonstrate that NAFLD significantly affects hair trace elements content in rats.

KEYWORDS: NAFLD, obesity, hair, trace elements, vanadium, zinc.