TRACE ELEMENTS IN MEDICINE (MOSCOW)
2016, 17(1): 38-44
ORIGINAL PAPERS

COMPARATIVE ANALYSIS OF INFORMATIVITY OF DIAGNOSTIC BIOSUBSTRATES (BLOOD SERUM AND DECKHAIR) WHEN DETERMINING ELEMENT STATUS OF EXPERIMENTAL ANIMALS

A.A. Skalny 1, M.V. Melikhova 1, E.Yu. Bonitenko 1, A.V. Skalny 1,2,4, M.G. Skalnaya 2,3, S.A. Miroshnikov 2,3

1 Institute of Toxicology of the Federal Medical-Biological Agency, Bekhtereva str. 1, St. Petersburg, 192019, Russia
2 Orenburg State University, Pobedy str. 13, Orenburg, 460015, Russia
3 All-Russian Research Institute of Beef Cattle Breeding, 9 Janvarja str. 29, Orenburg, 460000, Russia
4 All-Russian Research Institute of Medicinal and Aromatic Plants, Grina str. 7/1, Moscow, 117216, Russia

DOI: 10.19112/2413-6174-2016-17-1-38-44  

ABSTRACT. Informativity of diagnostic biosubstrates for determination of healthy nonlinear white male rats’ elemental status was studied. Ca, Mg, Fe, Zn, Cu, Mn and Se was determined in samples of blood serum, hair, liver, kidneys, myocardium and hip (thigh) muscle by ICP-MS. Direct correlations between the content of Ca, Zn, Cu in liver; Ca and Zn in kidneys; Ca in myocardial muscle and hair were found. On the basis of the correlation analysis of the found results we claim that the hair (fur) analysis of the content of Ca, Zn, Cu and Se is an informative method for the assessment of the status of these elements in the healthy body and reflects their active participation in metabolic processes (synthesis of proteins, excretion, detoxification and other functions). However, the analysis of blood serum to a lesser degree reflects the state of metabolism of trace elements comparing to hair: only the concentration of two elements - Cu and Zn in blood serum correlated with the content of these trace elements in kidneys and liver correspondingly. The potential of application of hair analysis as the method for control of redistribution of chemical elements between organs and tissues has been shown.

KEYWORDS: macro elements, trace elements, rats, hair, blood serum, liver, kidney, muscles.