ABSTRACT. Speciation analysis (analysis of chemical forms of elements) is an important field in analytical chemistry, which makes it possible to determine not only the total content of an element, but also its specific chemical forms (types). As a rule, two complementary methods are needed for speciation analysis of trace elements – separation and detection. The first ensures efficient and reliable separation of forms, while the second ensures adequate detection and quantification. This is especially important for understanding the bioavailability, toxicity, and environmental impact of the elements. The article considers the most modern, applied and available methods of separation of forms of some chemical elements. Progress in analytical tools and methodology makes it possible to identify and analyze the forms presented in a specific individual system.
KEYWORDS: speciation analysis, inductively coupled plasma mass spectrometry, high performance liquid chromatography, capillary electrophoresis, selenium, arsenic, mercury.
For citation: Lyapunovsky D.M. Separation methods for speciation analysis in biomedical research. Trace elemets in medicine. 2025;26(2):55-62. DOI: 10.19112/2413-6174-2025-26-2-55-62.
Information about the author:
D.M. Lyapunovsky – Post-graduate Student, Research Intern, Research Institute of Molecular and Cellular Medicine, Medical Institute; Leading Analytical Chemist; ORCID: 0009-0003-4863-1792