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Trace Elements in Medicine
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INFLUENCE OF HYDROGEN SULFIDE ON THE INDICATORS OF HEMOSTASIS, CALCIUM IONS AND THE DEVELOPMENT OF SIGNS OF SENSECTION IN THE VASCULAR NETWORK

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Trizno E.V., Bednov I.A., Trizno M.N., Davydova D.R., Shur V.Yu., O.S. Dyukareva

Astrakhan State Medical University of the Ministry of Health of the Russian Federation
121, Bakinskaya Str., Astrahan', 414000, Rossijskaya Federaciya

 

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ABSTRACT. Hydrogen sulfide (H2S) is considered an important regulatory gas transmitter required for hemovascular homeostasis. However, the physiological effect of its exposure varies greatly and depends on the concentration.

The aim of the work was to analyze the parameters of the hemostasis system and calcium ions (Ca++) in plasma before and after exposure to hydrogen sulfide.

Materials and methods. For expirement, venous blood and sections of the renal tissue and arteries of thirty-one laboratory rats were examined. The indicators of coagulation, fibrinolytic, anticoagulant systems and Ca++ -ions, as well as morphological changes in vascularized tissues were studied before and after exposure to the hydrogen sulfide donor sodium sulfide (Na2S).

Results. As a result of exposure to the pathogen, an increase in the level of Ca ++ ions in plasma, an acceleration of the time of formation of prothrombinase, to a greater extent along the external pathway, a slowdown in fibrinolysis, but an increase in the anticoagulant link, were recorded. Activated partial thromboplastin, prothrombin and thrombin time, antithrombin III, fibrinogen, and XIIa-dependent euglobulin lysis were studied as parameters of the hemostasis system. Among the cells of the endothelium and the medial vascular layer, senescent cells (SCs), multiple signs of degenerative disorders, and slugging of erythrocytes were observed. Lipofuscin-containing SCs were located diffusely in the smooth muscle vascular layer, and clusters were observed among the endothelial cells.

Conclusion. An excess of hydrogen sulfide ions promotes an increase in Ca++ -ions and the formation of senescent cells, which phenotypically modifies endotheliocytes and changes their regulatory function in relation to the hemostasis system.

KEYWORDS: ionized calcium, senescent cells, hemostasis, hydrogen sulfide donors, lipofuscin.