Trizno E.V., Bednov I.A., Trizno
M.N., Davydova D.R., Shur V.Yu., O.S. Dyukareva
Astrakhan State Medical University of
the Ministry of Health of the Russian Federation
121, Bakinskaya Str., Astrahan', 414000, Rossijskaya Federaciya
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ABSTRACT. Hydrogen sulfide (H2S) is considered an
important regulatory gas transmitter required for hemovascular homeostasis. However, the physiological effect of its exposure
varies greatly and depends on the concentration.
The aim of the work
was to analyze the parameters of the hemostasis system and calcium ions (Ca++)
in plasma before and after exposure to hydrogen sulfide.
Materials and methods. For expirement, venous blood and sections of the renal
tissue and arteries of thirty-one laboratory rats were examined. The indicators of coagulation, fibrinolytic,
anticoagulant systems and Ca++ -ions, as well as morphological
changes in vascularized tissues were studied before and after exposure to the
hydrogen sulfide donor sodium sulfide (Na2S).
Results. As
a result of exposure to the pathogen, an increase in the level of Ca ++
ions in plasma, an acceleration of the time of formation of prothrombinase, to
a greater extent along the external pathway, a slowdown in fibrinolysis, but an
increase in the anticoagulant link, were recorded. Activated
partial thromboplastin, prothrombin and thrombin time, antithrombin III,
fibrinogen, and XIIa-dependent euglobulin lysis were studied as parameters of
the hemostasis system. Among the cells of the
endothelium and the medial vascular layer, senescent cells (SCs), multiple
signs of degenerative disorders, and slugging of erythrocytes were observed. Lipofuscin-containing SCs were located diffusely in
the smooth muscle vascular layer, and clusters were observed among the
endothelial cells.
Conclusion. An
excess of hydrogen sulfide ions promotes an increase in Ca++ -ions
and the formation of senescent cells, which phenotypically modifies
endotheliocytes and changes their regulatory function in relation to the hemostasis
system.
KEYWORDS:
ionized calcium, senescent cells, hemostasis, hydrogen sulfide donors,
lipofuscin.