A.A. Shumakova1, A.I.
Kolobanov1, V.A. Shipelin1,2,
M.A. Ananyan3, I.V. Gmoshinski1*, S.A. Khotimchenko1,4
1 Federal Research Centre of Nutrition and
Biotechnology,
2/14 Ust’insky proezd, Moscow, 109240, Russia
2 Advanced Technologies Ltd,
4 Bardina ul., Moscow,119334, Russia
3 Plekhanov Russian University of Economics,
36 Stremyannyy per., Moscow, 115093, Russia
4 I.M. Sechenov First
Moscow State Medical University (Sechenov University),
8 str.2 Trubetskaya ul, Moscow, 119991, Russia
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ABSTRACT. The use of biologically active substances in
specialized food products is of interest in the dietary therapy of occupational
diseases caused by heavy metal intoxication. Dihydroquercetin
(3,5,7,3′,4′-pentahydroxy-flavanone, taxifolin, DHQ) has a powerful antioxidant
and antitoxic effect.
The aim of the work was to study the
influence of DHQ on the levels of trace elements in the organs of rats under
intoxication with nickel nanoparticles (Ni NPs).
Materials
and Methods.
Wistar rats received Ni NPs with average diameters of 53.7 ± 2.9 nm (Ni NP1)
and 70,9 ± 3.3 nm (Ni NP2) at a dose of 10 mg/kg of body weight (b.w.) for Ni
and a water-soluble stabilized form of DHQ "Taxifolin-aqua" with
drinking water at a dose of 23 mg/kg b.w. The content of Ni, as well as 26
chemical elements, including essential (Ca, Co, Cr, Cu, Fe, Mg, Mn, Se, Zn),
toxic (Al, As , Be, Ba, Cd, Pb, Sr, Tl) and with an unexplained function (Ag,
B, Ce, Cs, Ga, Gd, La, Rb, V) was measured by ICP-MS in the liver, kidney,
gonads and brain.
Results. DHQ intake led to a decrease
in Ni accumulation in the liver of rats treated with Ni NP 1. In the brain,
there was a decrease to baseline Ni accumulation in rats treated with Ni NP 2
together with DHQ. Among the elements, the levels of which exceeded the limit
of quantitative determination, in the liver DHQ led to a significant decrease
in Al, As, B, Ba, Sr, V and an increase in lead (Pb) in rats exposed to Ni NP1,
to a decrease in Ba, Sr and V at introduction of Ni NP2. In the kidneys, DHQ
caused a decrease in As, B, Pb, and V with an increase in Ba upon Ni NP1
administration, and a decrease in B and V upon Ni NP2. In the brain, DHA led to
a decrease in the bioaccumulation of B and Co and an increase in Pb with the
consumption of Ni NP1, a decrease in Al, B, Ba, and Co with Ni NP2.
Conclusion. DHQ had
multidirectional effects on the trace element homeostasis of rats treated with
Ni NPs, including such positive effects as the normalization of the level of Ni
in the brain and a decrease in the bioaccumulation of certain toxic elements -
Al, As, Ba, and V.
KEYWORDS: nanoparticles,
nickel, dihydroquercetin, rats, toxicity, bioaccumulation, trace elements.