Trace Elements in Medicine (Moscow)
2021, 22(2): 25-35
CHARACTERISTICS OF OXIDATIVE STRESS INDUCED BY MOD-ERATE DOSES OF SODIUM NITRITE IN ISOLATED ERYTHROCYTES IN THE PRESENCE OF SODIUM SELENITE
T.M. Huseynov*, S.Y. Huseynova, R.T. Guliyeva, M.Z. Dadashov, S.М. Rahmanova (Maharramova), F.R. Yakhyayeva, S.А. Jafarova
İnstitute of Biophysics of NAS, st. Z. Khalilov, 117, AZ1141, Baku, Azerbaijan
ABSTRACT. The effect of low and moderate sodium nitrite doses (final concentration 0.007, 0.070, 0.15, 0.35, 0.70 and 3.50 mM) on oxidative processes in erythrocytes, glutathione content, changes in catalase and glutathione peroxidase activity and the intensity of lipid peroxidation and the effect of sodium selenite (5 µM) as a possible antioxidant factor affecting the development of oxidative processes in vitro (incubation period 30 min, t = 37 °C) was considered. During nitrite-induced oxidation, there is a depletion of intra-erythrocyte glutatione (up to 12% of the initial level), which is in direct proportion to the final concentration of sodium nitrite. Catalase activity at all final sodium nitrite concentrations decreases, reaching a minimum already in the middle of the incubation period. In case of nitrite-induced oxidation, multidirectional changes in the glutathione peroxidase activity take place, which, at low and moderate sodium nitrite concentrations slightly increasein the initial period of incubation and decrease in the middle of the period. Catalase and glutathione peroxidase activity decrease in erythrocytes caused by nitrites is accompanied by an intensive accumulation of methemoglobin and an increase in lipid peroxidation at high concentrations of sodium nitrite (0,703,50 mM) by the end of the incubation period. The negative relationship between a decrease in glutathione peroxidase activity and an increase in lipid peroxidation intensity suggests a predominant role of glutathione peroxidase in limiting oxidative stress in erythrocytes.The results obtained indicate the active participation of these antioxidant erythrocytes enzymes in oxidative metabolism under nitrite exposure. Sodium selenite can, to a certain extent, act as a regulator of nitrite-induced oxidative stress.
КЛЮЧЕВЫЕ СЛОВА: erythrocytes, hemoglobin, sodium nitrite, sodium selenite, glutathione peroxidase, catalase, lipid peroxidation.Corresponding author: Tokay Huseynov E-mail: email@example.com